Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082691..2083193 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IHN50_RS10315 Protein ID WP_000767829.1
Coordinates 2082691..2082945 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IHN50_RS10320 Protein ID WP_001259255.1
Coordinates 2082942..2083193 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS10285 2077706..2077933 - 228 WP_000234896.1 sulfurtransferase TusA -
IHN50_RS10290 2077947..2079005 - 1059 WP_000492339.1 transport protein YeeE -
IHN50_RS10295 2079184..2080542 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IHN50_RS10300 2080532..2080594 - 63 WP_010723108.1 membrane protein YoeI -
IHN50_RS10305 2080809..2081738 - 930 WP_000803351.1 LysR family transcriptional regulator -
IHN50_RS10310 2081784..2082608 - 825 WP_000754737.1 SDR family oxidoreductase -
IHN50_RS10315 2082691..2082945 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IHN50_RS10320 2082942..2083193 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IHN50_RS10325 2083476..2083526 + 51 WP_001364200.1 his operon leader peptide -
IHN50_RS10330 2083672..2084571 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IHN50_RS10335 2084577..2085881 + 1305 WP_000009594.1 histidinol dehydrogenase -
IHN50_RS10340 2085878..2086948 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IHN50_RS10345 2086948..2088015 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T173914 WP_000767829.1 NZ_CP062245:c2082945-2082691 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173914 NZ_CP080383:2315045-2315290 [Cryobacterium sp. PAMC25264]
GTGGCAATTACAGCCTCACAAGCAAGGGCTCAGTTGTTCCCGTTGATCGAAAAGGTCAATGAGGACCACATGCCCATCGA
AATCGTCTCCAAGAAGGGCAGCGCGGTTCTCATGTCGCTGGCTGACTACGAGGCACTCGAAGAAACCGCCTACCTGCTGC
GTTCCCCCGCTAACGCGGCGCACCTGCTCGAAAGCATCGCGCAGCACCGGGCCGGCACGGCGAGCGTTCGTCCCCTGGCC
GAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173914 WP_001259255.1 NZ_CP062245:c2083193-2082942 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173914 NZ_CP080383:2315287-2315541 [Cryobacterium sp. PAMC25264]
GTGATAATCGCGTTCACGGATGCCGGCTGGGACGACTATCAATATTGGGTCACCACTGACCGGACGATGGTGAAACGCAT
CAACAAGCTCATCGCCGACGTGCAACGCACCCCGTTCACCGGCATCGGTAAACCCGAAGCGCTCAAGCATCAACTCGCCG
GGTACTGGTCCCGCCGCATCACCGATGAACACCGGCTGGTGTACACGGTGGAGCAGAACGAGATCGTCATCATCGCCGCT
CGGCATCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References