Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 248492..249186 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag IHN50_RS01170 Protein ID WP_001263489.1
Coordinates 248788..249186 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IHN50_RS01165 Protein ID WP_000554758.1
Coordinates 248492..248785 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS01145 244124..244621 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IHN50_RS01150 244845..246584 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
IHN50_RS01155 246529..247314 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IHN50_RS01160 247385..248440 + 1056 WP_001226164.1 DNA polymerase IV -
IHN50_RS01165 248492..248785 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IHN50_RS01170 248788..249186 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IHN50_RS01175 249196..249648 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
IHN50_RS01180 249966..250172 + 207 Protein_228 RtcB family protein -
IHN50_RS01185 250168..250689 + 522 Protein_229 peptide chain release factor H -
IHN50_RS01190 250746..252203 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
IHN50_RS01195 252464..252922 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -
- 253518..253598 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T173889 WP_001263489.1 NZ_CP062245:248788-249186 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T173889 NZ_CP080370:1111554-1111865 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT173889 WP_000554758.1 NZ_CP062245:248492-248785 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT173889 NZ_CP080370:1111862-1112281 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References