Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082692..2083194 Replicon chromosome
Accession NZ_CP062243
Organism Escherichia coli strain AML003_ev03

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IHN52_RS10315 Protein ID WP_000767829.1
Coordinates 2082692..2082946 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IHN52_RS10320 Protein ID WP_001259255.1
Coordinates 2082943..2083194 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN52_RS10285 2077707..2077934 - 228 WP_000234896.1 sulfurtransferase TusA -
IHN52_RS10290 2077948..2079006 - 1059 WP_000492339.1 transport protein YeeE -
IHN52_RS10295 2079185..2080543 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IHN52_RS10300 2080533..2080595 - 63 WP_010723108.1 membrane protein YoeI -
IHN52_RS10305 2080810..2081739 - 930 WP_000803351.1 LysR family transcriptional regulator -
IHN52_RS10310 2081785..2082609 - 825 WP_000754737.1 SDR family oxidoreductase -
IHN52_RS10315 2082692..2082946 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IHN52_RS10320 2082943..2083194 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IHN52_RS10325 2083477..2083527 + 51 WP_001364200.1 his operon leader peptide -
IHN52_RS10330 2083673..2084572 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IHN52_RS10335 2084578..2085882 + 1305 WP_000009594.1 histidinol dehydrogenase -
IHN52_RS10340 2085879..2086949 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
IHN52_RS10345 2086949..2088016 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T173828 WP_000767829.1 NZ_CP062243:c2082946-2082692 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173828 NZ_CP080333:c4330521-4330219 [Mycolicibacterium pallens]
GTGATCGCTCCCGGTGACATCGCGCCACGTCGCGATACCAGCCATGAGGTCTATGTTGTCGTGCTGTCTAACTCGATTCA
CCTGGCAGCCGATACCGGCCGAGTCATTACCTGCCCTTTTATTCCAGGCGAAATCCCCGACGCTGCAATGGCGTTGGTCG
TTCGAGTCGAACACCCCGAAGGCGTGGCGCTACCCGAGCTTGTTCAGTGGCTTCCCGTAGCAGCACTGGACGAGCCGATC
GGCAACATCGGGACGAACGCGCTAGGTGACACAGCGTCTCTCGTAACAGCCCTCGTCACCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173828 WP_001259255.1 NZ_CP062243:c2083194-2082943 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173828 NZ_CP080333:c4330745-4330518 [Mycolicibacterium pallens]
ATGGCGAAAGACAAGGTGTCGATCACACTCGATCAGGACGTGCTGGCAAAGGCCGATGCCGACGCCAAAGCCGCTGGGCT
CAACCGGTCCGAATTGGTTGAGCAGGCTTTGCGCCACGAGCACCTTCGGATCGCGCTCAAGAGCTACACATCGACGACCG
TCCCCGCGCTGAGCATCGACGATTACGCGCAGAGGATCTACCAGGCGAATCGGGCCTCGGGTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References