Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4359076..4359678 Replicon chromosome
Accession NZ_CP062228
Organism Escherichia coli strain UTI89

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag IFE20_RS21025 Protein ID WP_000897302.1
Coordinates 4359076..4359387 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IFE20_RS21030 Protein ID WP_000356397.1
Coordinates 4359388..4359678 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFE20_RS21000 4354990..4355589 + 600 WP_001314324.1 glucose-1-phosphatase -
IFE20_RS21005 4355583..4356455 + 873 WP_000920762.1 virulence factor BrkB family protein -
IFE20_RS21010 4356452..4356889 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
IFE20_RS21015 4356934..4357875 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
IFE20_RS21020 4357939..4358847 - 909 WP_001331553.1 alpha/beta hydrolase -
IFE20_RS21025 4359076..4359387 + 312 WP_000897302.1 hypothetical protein Toxin
IFE20_RS21030 4359388..4359678 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
IFE20_RS21035 4360037..4360315 + 279 WP_001296612.1 hypothetical protein -
IFE20_RS21040 4360711..4360929 + 219 WP_001314326.1 ribbon-helix-helix domain-containing protein -
IFE20_RS21045 4361145..4362074 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
IFE20_RS21050 4362071..4362706 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
IFE20_RS21055 4362703..4363605 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T173613 WP_000897302.1 NZ_CP062228:4359076-4359387 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T173613 NZ_CP080251:c2191220-2191116 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT173613 WP_000356397.1 NZ_CP062228:4359388-4359678 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT173613 NZ_CP080251:2191023-2191061 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References