Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2227618..2228120 Replicon chromosome
Accession NZ_CP062228
Organism Escherichia coli strain UTI89

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag IFE20_RS10855 Protein ID WP_000767822.1
Coordinates 2227618..2227872 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IFE20_RS10860 Protein ID WP_001259255.1
Coordinates 2227869..2228120 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFE20_RS10835 2224111..2225469 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
IFE20_RS10840 2225459..2225521 - 63 WP_010723108.1 membrane protein YoeI -
IFE20_RS10845 2225736..2226665 - 930 WP_000803366.1 LysR family transcriptional regulator -
IFE20_RS10850 2226711..2227535 - 825 WP_000754733.1 SDR family oxidoreductase -
IFE20_RS10855 2227618..2227872 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IFE20_RS10860 2227869..2228120 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IFE20_RS10865 2228403..2228453 + 51 WP_001364200.1 his operon leader peptide -
IFE20_RS10870 2228599..2229498 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IFE20_RS10875 2229504..2230808 + 1305 WP_001297912.1 histidinol dehydrogenase -
IFE20_RS10880 2230805..2231875 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
IFE20_RS10885 2231875..2232942 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T173610 WP_000767822.1 NZ_CP062228:c2227872-2227618 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173610 NZ_CP080251:c2019439-2019263 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173610 WP_001259255.1 NZ_CP062228:c2228120-2227869 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173610 NZ_CP080251:2019132-2019273 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References