Detailed information of TA system TA173576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3016843..3017429
• Replicon: chromosome
• Accession: NZ_CP062218
• Organism: Pseudomonas putida strain SY153

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A6I2K6L8
• Locus tag: IGB31_RS13825
• Protein ID: WP_029885077.1
• Coordinates: 3017151..3017429 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: IGB31_RS13820
• Protein ID: WP_029885078.1
• Coordinates: 3016843..3017142 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGB31_RS13795	3012729..3013538	+	810	WP_009686964.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
IGB31_RS13800	3013610..3014020	-	411	WP_054881203.1	SufE family protein	-
IGB31_RS13805	3014017..3015222	-	1206	WP_029885081.1	cysteine desulfurase	-
IGB31_RS13810	3015304..3016338	-	1035	WP_009686960.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
IGB31_RS13815	3016370..3016717	-	348	WP_054881204.1	ArsC family reductase	-
IGB31_RS13820	3016843..3017142	-	300	WP_029885078.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
IGB31_RS13825	3017151..3017429	-	279	WP_029885077.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IGB31_RS13830	3017678..3019324	+	1647	WP_029885076.1	Na+/H+ antiporter	-
IGB31_RS13835	3019868..3021064	-	1197	WP_029888073.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.72 Da
• Isoelectric Point: 7.1344

>T173576 WP_029885077.1 NZ_CP062218:c3017429-3017151 [Pseudomonas putida]
MIQSFSCADTEALFVTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLEALSGNRAGQHSIRVNDQWRLCFTWTD
NGPANVEIIDYH

Nucleotide

• Length: 279 bp

>T173576 NZ_CP080247:c1800393-1800019 [Escherichia sp. TM-G17TGC]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10903.58 Da
• Isoelectric Point: 6.4603

>AT173576 WP_029885078.1 NZ_CP062218:c3017142-3016843 [Pseudomonas putida]
MLKNGMRPIHPGEVLREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQRHGEEIIGAVQRLVA

Nucleotide

• Length: 300 bp

>AT173576 NZ_CP080247:c1800850-1800482 [Escherichia sp. TM-G17TGC]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A6I2K6L8