Detailed information of TA system TA173574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82878..83131
• Replicon: plasmid unnamed3
• Accession: NZ_CP062214
• Organism: Escherichia coli strain L3Cip3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IFO96_RS25900
• Protein ID: WP_001312851.1
• Coordinates: 82982..83131 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82878..82937 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFO96_RS25875	79605..80351	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IFO96_RS25880	80406..80966	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IFO96_RS25885	81098..81298	+	201	WP_015059022.1	hypothetical protein	-
IFO96_RS25890	81684..82283	+	600	WP_032083981.1	hypothetical protein	-
IFO96_RS25895	82447..82677	+	231	WP_001736714.1	hypothetical protein	-
-	82878..82937	-	60	NuclAT_1	-	Antitoxin
-	82878..82937	-	60	NuclAT_1	-	Antitoxin
-	82878..82937	-	60	NuclAT_1	-	Antitoxin
-	82878..82937	-	60	NuclAT_1	-	Antitoxin
IFO96_RS25900	82982..83131	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IFO96_RS25905	83415..83663	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IVa / aph(4)-Ia / floR / blaTEM-1B / rmtB / blaCTX-M-55	-	1..83974	83974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T173574 WP_001312851.1 NZ_CP062214:82982-83131 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T173574 NZ_CP080247:1788249-1788500 [Escherichia sp. TM-G17TGC]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT173574 NZ_CP062214:c82937-82878 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin