Detailed information of TA system TA173571

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40869..41138
• Replicon: plasmid unnamed3
• Accession: NZ_CP062214
• Organism: Escherichia coli strain L3Cip3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IFO96_RS25655
• Protein ID: WP_001312861.1
• Coordinates: 40980..41138 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40869..40934 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFO96_RS25625	36579..37106	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IFO96_RS25630	37164..37397	+	234	WP_000006003.1	DUF905 family protein	-
IFO96_RS25635	37458..39481	+	2024	Protein_49	ParB/RepB/Spo0J family partition protein	-
IFO96_RS25640	39550..39984	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IFO96_RS25645	39981..40700	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	40712..40936	+	225	NuclAT_0	-	-
-	40712..40936	+	225	NuclAT_0	-	-
-	40712..40936	+	225	NuclAT_0	-	-
-	40712..40936	+	225	NuclAT_0	-	-
IFO96_RS25650	40721..40900	-	180	WP_001309233.1	hypothetical protein	-
-	40869..40934	-	66	-	-	Antitoxin
IFO96_RS25655	40980..41138	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IFO96_RS25660	41376..41753	-	378	Protein_54	hypothetical protein	-
IFO96_RS25665	42053..42349	+	297	WP_001272251.1	hypothetical protein	-
IFO96_RS25670	42460..43281	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IFO96_RS25675	43578..44225	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IFO96_RS25680	44502..44885	+	384	WP_000124981.1	relaxosome protein TraM	-
IFO96_RS25685	45076..45762	+	687	WP_015059009.1	PAS domain-containing protein	-
IFO96_RS25690	45856..46083	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IVa / aph(4)-Ia / floR / blaTEM-1B / rmtB / blaCTX-M-55	-	1..83974	83974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T173571 WP_001312861.1 NZ_CP062214:40980-41138 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T173571 NZ_CP080247:972640-972975 [Escherichia sp. TM-G17TGC]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT173571 NZ_CP062214:c40934-40869 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin