Detailed information of TA system TA173560

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127877..128297
• Replicon: plasmid unnamed1
• Accession: NZ_CP062212
• Organism: Escherichia coli strain L3Cip3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: IFO96_RS24610
• Protein ID: WP_001302184.1
• Coordinates: 127877..128035 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128100..128297 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFO96_RS24570	122947..123162	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
IFO96_RS24575	123298..123945	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
IFO96_RS24580	124137..124520	-	384	WP_001063020.1	relaxosome protein TraM	-
IFO96_RS24585	124860..125462	+	603	WP_192301387.1	transglycosylase SLT domain-containing protein	-
IFO96_RS24590	125757..126578	-	822	WP_001234475.1	DUF945 domain-containing protein	-
IFO96_RS24595	126697..126984	-	288	WP_000107546.1	hypothetical protein	-
IFO96_RS24600	127009..127215	-	207	WP_000547965.1	hypothetical protein	-
IFO96_RS24605	127285..127640	+	356	Protein_125	hypothetical protein	-
IFO96_RS24610	127877..128035	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	128100..128297	-	198	NuclAT_0	-	Antitoxin
-	128100..128297	-	198	NuclAT_0	-	Antitoxin
-	128100..128297	-	198	NuclAT_0	-	Antitoxin
-	128100..128297	-	198	NuclAT_0	-	Antitoxin
IFO96_RS24615	128268..129029	-	762	Protein_127	plasmid SOS inhibition protein A	-
IFO96_RS24620	129026..129452	-	427	Protein_128	conjugation system SOS inhibitor PsiB	-
IFO96_RS24625	129507..131465	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
IFO96_RS24630	131531..131764	-	234	WP_000005990.1	DUF905 family protein	-
IFO96_RS24635	131821..132360	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
IFO96_RS24640	132678..133184	+	507	Protein_132	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..177038	177038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T173560 WP_001302184.1 NZ_CP062212:c128035-127877 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T173560 NZ_CP080246:3994717-3995094 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Nucleotide

• Length: 198 bp

>AT173560 NZ_CP062212:c128297-128100 [Escherichia coli]
ACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATGG
GCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGAC
AAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin