Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4899221..4899823 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag IFO96_RS23790 Protein ID WP_000897305.1
Coordinates 4899221..4899532 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IFO96_RS23795 Protein ID WP_000356397.1
Coordinates 4899533..4899823 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS23765 4895135..4895734 + 600 WP_001295269.1 glucose-1-phosphatase -
IFO96_RS23770 4895728..4896600 + 873 WP_000920762.1 virulence factor BrkB family protein -
IFO96_RS23775 4896597..4897034 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
IFO96_RS23780 4897079..4898020 + 942 WP_192301385.1 fatty acid biosynthesis protein FabY -
IFO96_RS23785 4898084..4898992 - 909 WP_001162704.1 alpha/beta hydrolase -
IFO96_RS23790 4899221..4899532 + 312 WP_000897305.1 hypothetical protein Toxin
IFO96_RS23795 4899533..4899823 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
IFO96_RS23800 4900409..4900627 + 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
IFO96_RS23805 4900846..4901088 + 243 WP_001087409.1 hypothetical protein -
IFO96_RS23810 4901418..4902347 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
IFO96_RS23815 4902344..4902979 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
IFO96_RS23820 4902976..4903878 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T173555 WP_000897305.1 NZ_CP062211:4899221-4899532 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T173555 NZ_CP080246:c3062647-3062540 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTTGGCTGGTGGCGTAGCCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT173555 WP_000356397.1 NZ_CP062211:4899533-4899823 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT173555 NZ_CP080246:3062703-3062764 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References