Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4114251..4115050 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag IFO96_RS20010 Protein ID WP_000347266.1
Coordinates 4114586..4115050 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IFO96_RS20005 Protein ID WP_001307405.1
Coordinates 4114251..4114586 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS19990 4110036..4110806 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IFO96_RS19995 4110822..4112156 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IFO96_RS20000 4112531..4114102 + 1572 WP_001273741.1 galactarate dehydratase -
IFO96_RS20005 4114251..4114586 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IFO96_RS20010 4114586..4115050 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IFO96_RS20015 4115105..4115914 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IFO96_RS20020 4116163..4117443 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
IFO96_RS20025 4117466..4117939 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IFO96_RS20030 4117950..4118729 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IFO96_RS20035 4118719..4119597 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IFO96_RS20040 4119615..4120049 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4102316..4115050 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T173553 WP_000347266.1 NZ_CP062211:4114586-4115050 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T173553 NZ_CP080246:c2212538-2212320 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT173553 WP_001307405.1 NZ_CP062211:4114251-4114586 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT173553 NZ_CP080246:c2212938-2212564 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References