Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2858721..2859223 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag IFO96_RS13925 Protein ID WP_000767820.1
Coordinates 2858721..2858975 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IFO96_RS13930 Protein ID WP_001259255.1
Coordinates 2858972..2859223 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS13895 2853736..2853963 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
IFO96_RS13900 2853977..2855035 - 1059 WP_000492346.1 transport protein YeeE -
IFO96_RS13905 2855214..2856572 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IFO96_RS13910 2856562..2856624 - 63 WP_010723108.1 membrane protein YoeI -
IFO96_RS13915 2856839..2857768 - 930 WP_000803351.1 LysR family transcriptional regulator -
IFO96_RS13920 2857814..2858638 - 825 WP_000754758.1 SDR family oxidoreductase -
IFO96_RS13925 2858721..2858975 - 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IFO96_RS13930 2858972..2859223 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IFO96_RS13935 2859507..2859557 + 51 WP_001364200.1 his operon leader peptide -
IFO96_RS13940 2859703..2860602 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IFO96_RS13945 2860608..2861912 + 1305 WP_000009586.1 histidinol dehydrogenase -
IFO96_RS13950 2861909..2862979 + 1071 WP_000108928.1 histidinol-phosphate transaminase -
IFO96_RS13955 2862979..2864046 + 1068 WP_000080140.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T173549 WP_000767820.1 NZ_CP062211:c2858975-2858721 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173549 NZ_CP080246:1206673-1206984 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173549 WP_001259255.1 NZ_CP062211:c2859223-2858972 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173549 NZ_CP080246:1206981-1207400 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References