Detailed information of TA system TA173529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8825..9471
• Replicon: plasmid p2
• Accession: NZ_CP062206
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IG172_RS25940
• Protein ID: WP_000269912.1
• Coordinates: 9124..9471 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: IG172_RS25935
• Protein ID: WP_001259436.1
• Coordinates: 8825..9124 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS25905	4428..4676	+	249	WP_000730008.1	hypothetical protein	-
IG172_RS25910	5501..5695	+	195	WP_157836022.1	ash family protein	-
IG172_RS25915	5806..5997	+	192	WP_000183351.1	hypothetical protein	-
IG172_RS25920	5994..7136	+	1143	WP_136828299.1	ORF6N domain-containing protein	-
IG172_RS25925	7946..8218	-	273	WP_192292591.1	helix-turn-helix domain-containing protein	-
IG172_RS25930	8282..8662	-	381	WP_000061763.1	hypothetical protein	-
IG172_RS25935	8825..9124	-	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
IG172_RS25940	9124..9471	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG172_RS25945	9716..9979	-	264	WP_000424604.1	hypothetical protein	-
IG172_RS25950	10003..10290	-	288	WP_000356589.1	hypothetical protein	-
IG172_RS25955	10934..11770	-	837	WP_024189035.1	replication initiator protein RepA	-
IG172_RS25960	12096..12650	-	555	WP_159379166.1	recombinase family protein	-
IG172_RS25965	12732..13700	+	969	WP_158160466.1	phage tail protein	-
IG172_RS25970	13703..14236	+	534	WP_097509664.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48103	48103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T173529 WP_000269912.1 NZ_CP062206:c9471-9124 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T173529 NZ_CP080235:c4527247-4527029 [Escherichia coli O139:H1]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT173529 WP_001259436.1 NZ_CP062206:c9124-8825 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT173529 NZ_CP080235:c4527647-4527273 [Escherichia coli O139:H1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1V3UZT0