Detailed information of TA system TA173523

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99118..99544
• Replicon: plasmid p1
• Accession: NZ_CP062205
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IG172_RS25630
• Protein ID: WP_001312861.1
• Coordinates: 99118..99276 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	99320..99544 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS25590	94178..94393	-	216	WP_077888289.1	conjugal transfer relaxosome protein TraY	-
IG172_RS25595	94529..95176	-	648	WP_069907063.1	conjugal transfer transcriptional regulator TraJ	-
IG172_RS25600	95357..95749	-	393	WP_192292517.1	relaxosome protein TraM	-
IG172_RS25605	96025..96672	+	648	WP_139124686.1	transglycosylase SLT domain-containing protein	-
IG172_RS25610	96969..97790	-	822	WP_001234469.1	DUF945 domain-containing protein	-
IG172_RS25615	97910..98107	-	198	WP_052318758.1	hypothetical protein	-
IG172_RS25620	98221..98427	-	207	WP_069907064.1	single-stranded DNA-binding protein	-
IG172_RS25625	98340..98675	-	336	WP_192292570.1	hypothetical protein	-
IG172_RS25630	99118..99276	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	99320..99544	-	225	NuclAT_0	-	Antitoxin
-	99320..99544	-	225	NuclAT_0	-	Antitoxin
-	99320..99544	-	225	NuclAT_0	-	Antitoxin
-	99320..99544	-	225	NuclAT_0	-	Antitoxin
IG172_RS25635	99356..99544	+	189	WP_001299721.1	hypothetical protein	-
IG172_RS25640	99556..100275	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
IG172_RS25645	100272..100706	-	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
IG172_RS25650	100775..102739	-	1965	WP_192292520.1	ParB/RepB/Spo0J family partition protein	-
IG172_RS25655	102804..103037	-	234	WP_000006030.1	DUF905 family protein	-
IG172_RS25660	103099..103674	-	576	WP_192292523.1	single-stranded DNA-binding protein	-
IG172_RS25665	103700..103906	-	207	WP_192292526.1	single-stranded DNA-binding protein	-
IG172_RS25670	103819..104154	-	336	WP_192292573.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..144932	144932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T173523 WP_001312861.1 NZ_CP062205:c99276-99118 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T173523 NZ_CP080235:3399974-3400285 [Escherichia coli O139:H1]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT173523 NZ_CP062205:c99544-99320 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin