Detailed information of TA system TA173522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4964586..4965188
• Replicon: chromosome
• Accession: NZ_CP062204
• Organism: Escherichia coli strain C311

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IG172_RS24160
• Protein ID: WP_000897305.1
• Coordinates: 4964877..4965188 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IG172_RS24155
• Protein ID: WP_000356397.1
• Coordinates: 4964586..4964876 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG172_RS24130	4960531..4961433	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IG172_RS24135	4961430..4962065	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IG172_RS24140	4962062..4962991	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IG172_RS24145	4963321..4963563	-	243	WP_001087409.1	hypothetical protein	-
IG172_RS24150	4963782..4964000	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IG172_RS24155	4964586..4964876	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IG172_RS24160	4964877..4965188	-	312	WP_000897305.1	hypothetical protein	Toxin
IG172_RS24165	4965417..4966325	+	909	WP_001162704.1	alpha/beta hydrolase	-
IG172_RS24170	4966389..4967330	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IG172_RS24175	4967375..4967812	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IG172_RS24180	4967809..4968681	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IG172_RS24185	4968675..4969274	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T173522 WP_000897305.1 NZ_CP062204:c4965188-4964877 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T173522 NZ_CP080235:2567494-2567958 [Escherichia coli O139:H1]
ATGGATTTTCCACAAAGGGTTAATAGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCGATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT173522 WP_000356397.1 NZ_CP062204:c4964876-4964586 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT173522 NZ_CP080235:2567159-2567494 [Escherichia coli O139:H1]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7