Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1909517..1910019 Replicon chromosome
Accession NZ_CP062204
Organism Escherichia coli strain C311

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag IG172_RS09225 Protein ID WP_000767828.1
Coordinates 1909765..1910019 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IG172_RS09220 Protein ID WP_001259255.1
Coordinates 1909517..1909768 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG172_RS09195 1904695..1905762 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IG172_RS09200 1905762..1906832 - 1071 WP_001564244.1 histidinol-phosphate transaminase -
IG172_RS09205 1906829..1908133 - 1305 WP_001493159.1 histidinol dehydrogenase -
IG172_RS09210 1908139..1909038 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IG172_RS09215 1909184..1909234 - 51 WP_001364200.1 his operon leader peptide -
IG172_RS09220 1909517..1909768 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IG172_RS09225 1909765..1910019 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IG172_RS09230 1910102..1910926 + 825 WP_000754758.1 SDR family oxidoreductase -
IG172_RS09235 1910972..1911901 + 930 WP_000803351.1 LysR family transcriptional regulator -
IG172_RS09240 1912116..1912178 + 63 WP_010723108.1 membrane protein YoeI -
IG172_RS09245 1912168..1913526 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IG172_RS09250 1913705..1914763 + 1059 WP_192292293.1 transport protein YeeE -
IG172_RS09255 1914777..1915004 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T173505 WP_000767828.1 NZ_CP062204:1909765-1910019 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173505 NZ_CP080233:c3128895-3128644 [Escherichia coli O139:H1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173505 WP_001259255.1 NZ_CP062204:1909517-1909768 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173505 NZ_CP080233:c3128647-3128393 [Escherichia coli O139:H1]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References