Detailed information of TA system TA173499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4811048..4811650
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IGX36_RS23415
• Protein ID: WP_000897305.1
• Coordinates: 4811339..4811650 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IGX36_RS23410
• Protein ID: WP_000356395.1
• Coordinates: 4811048..4811338 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS23375	4806672..4807574	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IGX36_RS23380	4807571..4808206	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IGX36_RS23385	4808203..4809132	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IGX36_RS23390	4809314..4809556	-	243	WP_001306649.1	hypothetical protein	-
IGX36_RS23395	4809775..4809992	-	218	Protein_4573	CopG family transcriptional regulator	-
IGX36_RS23400	4810411..4810689	-	279	WP_001306650.1	hypothetical protein	-
IGX36_RS23405	4810751..4810963	-	213	WP_000197769.1	hypothetical protein	-
IGX36_RS23410	4811048..4811338	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
IGX36_RS23415	4811339..4811650	-	312	WP_000897305.1	hypothetical protein	Toxin
IGX36_RS23420	4811879..4812787	+	909	WP_001306651.1	alpha/beta hydrolase	-
IGX36_RS23425	4812956..4814770	-	1815	WP_000226339.1	hypothetical protein	-
IGX36_RS23430	4815186..4816127	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IGX36_RS23435	4816172..4816609	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T173499 WP_000897305.1 NZ_CP062203:c4811650-4811339 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T173499 NZ_CP080233:c2386279-2386085 [Escherichia coli O139:H1]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT173499 WP_000356395.1 NZ_CP062203:c4811338-4811048 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT173499 NZ_CP080233:2385908-2386086 [Escherichia coli O139:H1]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGCTGGTAGGAGAACCACCTTCCTTTT
CAATAGTGGCGGTGATTTT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7