Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3939897..3940591 Replicon chromosome
Accession NZ_CP062203
Organism Escherichia coli strain K_EC180

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag IGX36_RS19195 Protein ID WP_001521903.1
Coordinates 3939897..3940295 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag IGX36_RS19200 Protein ID WP_000554755.1
Coordinates 3940298..3940591 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGX36_RS19165 3935031..3936488 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
IGX36_RS19170 3936497..3936778 + 282 WP_077881290.1 hypothetical protein -
IGX36_RS19175 3936795..3937304 - 510 WP_063085380.1 metal-dependent hydrolase -
IGX36_RS19180 3937366..3937980 - 615 WP_000602129.1 peptide chain release factor H -
IGX36_RS19185 3937977..3939116 - 1140 WP_063085369.1 RNA ligase RtcB family protein -
IGX36_RS19190 3939435..3939887 - 453 WP_001059858.1 GNAT family N-acetyltransferase -
IGX36_RS19195 3939897..3940295 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IGX36_RS19200 3940298..3940591 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IGX36_RS19205 3940643..3941698 - 1056 WP_192297422.1 DNA polymerase IV -
IGX36_RS19210 3941769..3942554 - 786 WP_074150236.1 putative lateral flagellar export/assembly protein LafU -
IGX36_RS19215 3942499..3944238 + 1740 WP_192297424.1 flagellar biosynthesis protein FlhA -
IGX36_RS19220 3944343..3944621 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IGX36_RS19225 3944614..3944970 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T173495 WP_001521903.1 NZ_CP062203:c3940295-3939897 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T173495 NZ_CP080233:c1735016-1734630 [Escherichia coli O139:H1]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT173495 WP_000554755.1 NZ_CP062203:c3940591-3940298 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT173495 NZ_CP080233:c1734640-1734311 [Escherichia coli O139:H1]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATATCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References