Detailed information of TA system TA173480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1136698..1137425
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: IGX36_RS05400
• Protein ID: WP_000547555.1
• Coordinates: 1136698..1137009 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IGX36_RS05405
• Protein ID: WP_000126294.1
• Coordinates: 1137006..1137425 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS05375	1132633..1134342	+	1710	WP_063086195.1	formate hydrogenlyase subunit HycE	-
IGX36_RS05380	1134352..1134894	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
IGX36_RS05385	1134894..1135661	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
IGX36_RS05390	1135658..1136068	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
IGX36_RS05395	1136061..1136531	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IGX36_RS05400	1136698..1137009	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
IGX36_RS05405	1137006..1137425	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
IGX36_RS05410	1137504..1138928	-	1425	WP_063086210.1	6-phospho-beta-glucosidase AscB	-
IGX36_RS05415	1138937..1140394	-	1458	WP_001107888.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IGX36_RS05420	1140654..1141664	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
IGX36_RS05425	1141813..1142340	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T173480 WP_000547555.1 NZ_CP062203:1136698-1137009 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T173480 NZ_CP062203:1136698-1137009 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT173480 WP_000126294.1 NZ_CP062203:1137006-1137425 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT173480 NZ_CP062203:1137006-1137425 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2