Detailed information of TA system TA173477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 616312..617111
• Replicon: chromosome
• Accession: NZ_CP062203
• Organism: Escherichia coli strain K_EC180

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3A6SBL4
• Locus tag: IGX36_RS02935
• Protein ID: WP_062875446.1
• Coordinates: 616312..616776 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: IGX36_RS02940
• Protein ID: WP_001307405.1
• Coordinates: 616776..617111 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGX36_RS02905	611313..611747	-	435	WP_063086247.1	PTS sugar transporter subunit IIA	-
IGX36_RS02910	611765..612643	-	879	WP_192297513.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IGX36_RS02915	612633..613412	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IGX36_RS02920	613423..613896	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IGX36_RS02925	613919..615199	-	1281	WP_063086249.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IGX36_RS02930	615448..616257	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
IGX36_RS02935	616312..616776	-	465	WP_062875446.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IGX36_RS02940	616776..617111	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IGX36_RS02945	617260..618831	-	1572	WP_001273756.1	galactarate dehydratase	-
IGX36_RS02950	619206..620540	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
IGX36_RS02955	620556..621331	+	776	Protein_577	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17844.22 Da
• Isoelectric Point: 9.4942

>T173477 WP_062875446.1 NZ_CP062203:c616776-616312 [Escherichia coli]
MDFPQRVNSWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETD

Nucleotide

• Length: 465 bp

>T173477 NZ_CP080223:4477569-4477946 [Escherichia coli O141:H4]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT173477 WP_001307405.1 NZ_CP062203:c617111-616776 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT173477 NZ_CP080223:4477111-4477479 [Escherichia coli O141:H4]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGACCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3A6SBL4

Antitoxin

• AlphaFold DB: A0A0E0XVC7