Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3506333..3507128 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag IEM32_RS17365 Protein ID WP_000854914.1
Coordinates 3506333..3506707 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag IEM32_RS17370 Protein ID WP_001280954.1
Coordinates 3506754..3507128 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS17325 3501337..3501828 - 492 WP_001301587.1 DUF1097 domain-containing protein -
IEM32_RS17330 3501930..3502484 - 555 WP_001001917.1 molecular chaperone YcdY -
IEM32_RS17335 3502508..3503245 - 738 WP_000283664.1 zinc-binding phosphatase -
IEM32_RS17340 3503300..3504238 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
IEM32_RS17350 3504709..3505551 - 843 WP_001280481.1 DUF4942 domain-containing protein -
IEM32_RS17355 3505636..3505833 - 198 WP_000839282.1 DUF957 domain-containing protein -
IEM32_RS17360 3505845..3506336 - 492 WP_000976853.1 hypothetical protein -
IEM32_RS17365 3506333..3506707 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
IEM32_RS17370 3506754..3507128 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IEM32_RS17375 3507291..3507512 - 222 WP_001220314.1 DUF987 domain-containing protein -
IEM32_RS17380 3507575..3508051 - 477 WP_001186738.1 RadC family protein -
IEM32_RS17385 3508067..3508552 - 486 WP_000214398.1 antirestriction protein -
IEM32_RS17390 3508643..3509461 - 819 WP_001234682.1 DUF945 domain-containing protein -
IEM32_RS17395 3509801..3510871 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
IEM32_RS17400 3510868..3511773 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3497719..3508552 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T173454 WP_000854914.1 NZ_CP062160:c3506707-3506333 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T173454 NZ_CP080222:1635405-1635866 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT173454 WP_001280954.1 NZ_CP062160:c3507128-3506754 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT173454 NZ_CP080222:1635063-1635392 [Staphylococcus aureus]
ATGAGAAACAATGATGAAATAATCACAATAATCAAAACATCTATGAAAGAACAGAATTTATCACTTAGTGAATTGGCTCG
TCGTGTAGGAGTTGCTAAATCTGCTGTGTCACGTTATTTAAATTTAACGAGAGAATTTCCATTAAATAGAGCAGAAGATT
TCGCAAAAGCGCTTAGTATCAGCACAGAATATTTACTTGGTTTTGAAAAAAGTGAACAAAAACAAGAAGAACCACAGCAC
CGTGCAGCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAA
ACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References