Detailed information of TA system TA173436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1372961..1373655
• Replicon: chromosome
• Accession: NZ_CP062160
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: IEM32_RS06455
• Protein ID: WP_001263495.1
• Coordinates: 1373257..1373655 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IEM32_RS06450
• Protein ID: WP_000554757.1
• Coordinates: 1372961..1373254 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS06430	1368672..1369169	+	498	Protein_1242	REP-associated tyrosine transposase RayT	-
IEM32_RS06435	1369314..1371053	-	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
IEM32_RS06440	1370998..1371783	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
IEM32_RS06445	1371854..1372909	+	1056	WP_001226188.1	DNA polymerase IV	-
IEM32_RS06450	1372961..1373254	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IEM32_RS06455	1373257..1373655	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IEM32_RS06460	1373665..1374117	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
IEM32_RS06465	1374436..1374639	+	204	Protein_1249	RNA ligase RtcB family protein	-
IEM32_RS06470	1374638..1375159	+	522	Protein_1250	peptide chain release factor H	-
IEM32_RS06475	1375216..1376673	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
IEM32_RS06480	1376934..1377392	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1377988..1378068	+	81	NuclAT_12	-	-
-	1377988..1378068	+	81	NuclAT_12	-	-
-	1377988..1378068	+	81	NuclAT_12	-	-
-	1377988..1378068	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T173436 WP_001263495.1 NZ_CP062160:1373257-1373655 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T173436 NZ_CP080172:c3452840-3452505 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT173436 WP_000554757.1 NZ_CP062160:1372961-1373254 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT173436 NZ_CP080172:c3453088-3452840 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGAGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6