Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 107946..108567 Replicon plasmid pJAB4138
Accession NZ_CP062064
Organism Janthinobacterium sp. HH102

Toxin (Protein)


Gene name graT Uniprot ID A0A853SQY1
Locus tag JAB4_RS30015 Protein ID WP_070223560.1
Coordinates 108298..108567 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A853SQF0
Locus tag JAB4_RS30010 Protein ID WP_070223558.1
Coordinates 107946..108248 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JAB4_RS29980 104524..104928 + 405 WP_193725224.1 flagellar basal body rod protein FlgB -
JAB4_RS29985 105125..105439 + 315 WP_071650251.1 zinc ribbon domain-containing protein -
JAB4_RS29990 105548..105745 + 198 Protein_96 CopG family transcriptional regulator -
JAB4_RS29995 105745..105962 + 218 Protein_97 type II toxin-antitoxin system RelE/ParE family toxin -
JAB4_RS30000 106608..106787 + 180 WP_070223554.1 recombinase family protein -
JAB4_RS30005 106819..107511 + 693 WP_083287490.1 IS6 family transposase -
JAB4_RS30010 107946..108248 - 303 WP_070223558.1 HigA family addiction module antidote protein Antitoxin
JAB4_RS30015 108298..108567 - 270 WP_070223560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JAB4_RS30020 108687..109934 - 1248 WP_070223562.1 tyrosine-type recombinase/integrase -
JAB4_RS30025 109931..110929 - 999 WP_131687624.1 hypothetical protein -
JAB4_RS30030 111429..111593 - 165 WP_083287491.1 transposase -
JAB4_RS30035 111812..112054 - 243 WP_071650252.1 hypothetical protein -
JAB4_RS30040 112339..113151 - 813 WP_070223566.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..137594 137594


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-89)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-64)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10175.51 Da        Isoelectric Point: 7.1644

>T173368 WP_070223560.1 NZ_CP062064:c108567-108298 [Janthinobacterium sp. HH102]
MPIQSFACPDTQALFTTGRSRRFVNIKNVAERKLAQLDAAPSVNFMKAPPGNDLKEYDGAWHVRINDQWRLTFKWGDSGP
YEVLIEDPH

Download         Length: 270 bp

>T173368 NZ_CP080155:999861-1000268 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10942.76 Da        Isoelectric Point: 9.8337

>AT173368 WP_070223558.1 NZ_CP062064:c108248-107946 [Janthinobacterium sp. HH102]
MFKNGMRPIHPGEILVEDYIKPMGVSVRAVSLALHVPYSRLSEITKGERGVSADTALRLERYFGSEAQGWLNLQSAYDLR
MAEISAGKAIAKAIKPLALA

Download         Length: 303 bp

>AT173368 NZ_CP080155:999614-999880 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853SQY1

Antitoxin

Source ID Structure
AlphaFold DB A0A853SQF0

References