Detailed information of TA system TA173354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40486..41213
• Replicon: plasmid unnamed2
• Accession: NZ_CP061977
• Organism: Klebsiella pneumoniae strain IR5086

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: H4I67_RS25880
• Protein ID: WP_011251285.1
• Coordinates: 40486..40797 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4I67_RS25885
• Protein ID: WP_011251286.1
• Coordinates: 40794..41213 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I67_RS25850	35717..36697	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
H4I67_RS25855	37242..37421	-	180	Protein_37	IS5/IS1182 family transposase	-
H4I67_RS25860	37461..38999	-	1539	WP_032430751.1	IS66 family transposase	-
H4I67_RS25865	39048..39395	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
H4I67_RS25870	39392..39730	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
H4I67_RS25875	39823..40281	+	459	Protein_41	DDE-type integrase/transposase/recombinase	-
H4I67_RS25880	40486..40797	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
H4I67_RS25885	40794..41213	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
H4I67_RS25890	41384..42352	-	969	WP_016151349.1	IS5 family transposase	-
H4I67_RS25895	42899..43468	+	570	WP_064160296.1	type 1 fimbrial protein	-
H4I67_RS25900	43527..44222	+	696	WP_064160295.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA	1..266729	266729
	-	inside	IScluster/Tn	-	-	35717..42352	6635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T173354 WP_011251285.1 NZ_CP061977:40486-40797 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T173354 NZ_CP080146:4721884-4722180 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT173354 WP_011251286.1 NZ_CP061977:40794-41213 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT173354 NZ_CP080146:4722182-4722577 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1