Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330921..331507 Replicon chromosome
Accession NZ_CP061975
Organism Klebsiella pneumoniae strain IR5086

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag H4I67_RS01530 Protein ID WP_002920800.1
Coordinates 331139..331507 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag H4I67_RS01525 Protein ID WP_004174006.1
Coordinates 330921..331142 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I67_RS01505 327078..328004 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H4I67_RS01510 328001..329278 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
H4I67_RS01515 329275..330042 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
H4I67_RS01520 330044..330757 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
H4I67_RS01525 330921..331142 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H4I67_RS01530 331139..331507 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H4I67_RS01535 331780..333096 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
H4I67_RS01540 333203..334090 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
H4I67_RS01545 334087..334932 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
H4I67_RS01550 334934..336004 + 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 328001..336741 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T173335 WP_002920800.1 NZ_CP061975:331139-331507 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T173335 NZ_CP080143:c31217-30951 [Escherichia coli]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTGATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT173335 WP_004174006.1 NZ_CP061975:330921-331142 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT173335 NZ_CP080143:c30961-30719 [Escherichia coli]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTTCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References