Detailed information of TA system TA173298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1185528..1186125
• Replicon: chromosome
• Accession: NZ_CP061965
• Organism: Klebsiella pneumoniae strain LZK001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: H4I72_RS05710
• Protein ID: WP_004142563.1
• Coordinates: 1185528..1185845 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4I72_RS05715
• Protein ID: WP_076027550.1
• Coordinates: 1185838..1186125 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I72_RS05695	1181232..1182659	-	1428	WP_004176980.1	MFS transporter	-
H4I72_RS05700	1182768..1183634	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
H4I72_RS05705	1184300..1185343	+	1044	WP_048336957.1	DUF2157 domain-containing protein	-
H4I72_RS05710	1185528..1185845	+	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H4I72_RS05715	1185838..1186125	+	288	WP_076027550.1	helix-turn-helix transcriptional regulator	Antitoxin
H4I72_RS05720	1186389..1187042	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
H4I72_RS05725	1187162..1187530	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
H4I72_RS05730	1187530..1187685	-	156	Protein_1126	TetR/AcrR family transcriptional regulator	-
H4I72_RS05735	1187703..1188400	-	698	Protein_1127	IS1 family transposase	-
H4I72_RS05740	1188456..1188809	-	354	Protein_1128	sugar ABC transporter ATP-binding protein	-
H4I72_RS05745	1188811..1189431	-	621	WP_002893596.1	DUF2291 domain-containing protein	-
H4I72_RS05750	1189475..1190410	-	936	WP_012737614.1	D-ribose ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T173298 WP_004142563.1 NZ_CP061965:1185528-1185845 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T173298 NZ_CP080133:c4135198-4135025 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10783.51 Da
• Isoelectric Point: 10.2212

>AT173298 WP_076027550.1 NZ_CP061965:1185838-1186125 [Klebsiella pneumoniae]
MSKKIIDWDTLKIELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT173298 NZ_CP080133:c4135522-4135226 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8