Detailed information of TA system TA173296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 358433..359079
• Replicon: chromosome
• Accession: NZ_CP061965
• Organism: Klebsiella pneumoniae strain LZK001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A483JFR4
• Locus tag: H4I72_RS01645
• Protein ID: WP_004188313.1
• Coordinates: 358433..358780 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4I72_RS01650
• Protein ID: WP_004210373.1
• Coordinates: 358780..359079 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I72_RS01635	354369..355802	+	1434	WP_002920564.1	glycogen synthase GlgA	-
H4I72_RS01640	355820..358267	+	2448	WP_002920561.1	glycogen phosphorylase	-
H4I72_RS01645	358433..358780	+	348	WP_004188313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H4I72_RS01650	358780..359079	+	300	WP_004210373.1	helix-turn-helix transcriptional regulator	Antitoxin
H4I72_RS01655	359142..360650	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
H4I72_RS01660	360855..361184	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
H4I72_RS01665	361235..362065	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
H4I72_RS01670	362115..362873	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13548.57 Da
• Isoelectric Point: 6.2327

>T173296 WP_004188313.1 NZ_CP061965:358433-358780 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T173296 NZ_CP080133:3423214-3423432 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT173296 WP_004210373.1 NZ_CP061965:358780-359079 [Klebsiella pneumoniae]
MVTLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT173296 NZ_CP080133:3422814-3423188 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A483JFR4