Detailed information of TA system TA173270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90027..90296
• Replicon: plasmid unnamed3
• Accession: NZ_CP061960
• Organism: Klebsiella pneumoniae strain IR5726

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H4I74_RS28420
• Protein ID: WP_001312861.1
• Coordinates: 90138..90296 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90027..90092 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I74_RS28395	85737..86264	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H4I74_RS28400	86322..86555	+	234	WP_000006003.1	DUF905 family protein	-
H4I74_RS28405	86616..88639	+	2024	Protein_118	ParB/RepB/Spo0J family partition protein	-
H4I74_RS28410	88708..89142	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H4I74_RS28415	89139..89858	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	89870..90094	+	225	NuclAT_0	-	-
-	89870..90094	+	225	NuclAT_0	-	-
-	89870..90094	+	225	NuclAT_0	-	-
-	89870..90094	+	225	NuclAT_0	-	-
-	90027..90092	-	66	-	-	Antitoxin
H4I74_RS28420	90138..90296	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H4I74_RS28425	90534..90911	-	378	Protein_122	hypothetical protein	-
H4I74_RS28430	91262..91966	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H4I74_RS28435	92029..92424	+	396	Protein_124	ATP-dependent endonuclease	-
H4I74_RS28440	92409..93431	+	1023	WP_000361404.1	helicase UvrD	-
H4I74_RS28445	93687..94384	+	698	Protein_126	IS1 family transposase	-
H4I74_RS28450	94413..94685	+	273	Protein_127	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..144349	144349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T173270 WP_001312861.1 NZ_CP061960:90138-90296 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T173270 NZ_CP080126:3496660-3497202 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT173270 NZ_CP061960:c90092-90027 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin