Detailed information of TA system TA173266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68722..68975
• Replicon: plasmid unnamed3
• Accession: NZ_CP061960
• Organism: Klebsiella pneumoniae strain IR5726

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H4I74_RS28295
• Protein ID: WP_001312851.1
• Coordinates: 68826..68975 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68722..68781 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I74_RS28250	63777..63989	-	213	WP_014343500.1	hypothetical protein	-
H4I74_RS28255	64050..64406	-	357	WP_014343501.1	hypothetical protein	-
H4I74_RS28260	64500..65204	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H4I74_RS28265	65268..65429	+	162	Protein_90	DNA helicase	-
H4I74_RS28270	65449..66195	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H4I74_RS28275	66250..66810	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H4I74_RS28280	66942..67142	+	201	WP_015059022.1	hypothetical protein	-
H4I74_RS28285	67528..68127	+	600	WP_032083981.1	hypothetical protein	-
H4I74_RS28290	68291..68521	+	231	WP_001736714.1	hypothetical protein	-
-	68722..68781	-	60	NuclAT_1	-	Antitoxin
-	68722..68781	-	60	NuclAT_1	-	Antitoxin
-	68722..68781	-	60	NuclAT_1	-	Antitoxin
-	68722..68781	-	60	NuclAT_1	-	Antitoxin
H4I74_RS28295	68826..68975	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H4I74_RS28300	69259..69507	+	249	WP_206971190.1	replication regulatory protein RepA	-
H4I74_RS28305	69752..69826	+	75	WP_001375168.1	RepA leader peptide Tap	-
H4I74_RS28310	69819..70676	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
H4I74_RS28315	71615..72268	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H4I74_RS28320	72361..72618	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H4I74_RS28325	72551..72952	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H4I74_RS28330	73201..73616	+	416	Protein_103	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..144349	144349
	-	flank	IS/Tn	-	-	64500..65204	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T173266 WP_001312851.1 NZ_CP061960:68826-68975 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T173266 NZ_CP080126:2287082-2288404 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT173266 NZ_CP061960:c68781-68722 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin