Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331460..332046 Replicon chromosome
Accession NZ_CP061957
Organism Klebsiella pneumoniae strain IR5726

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag H4I74_RS01535 Protein ID WP_002920800.1
Coordinates 331678..332046 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag H4I74_RS01530 Protein ID WP_002920802.1
Coordinates 331460..331681 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I74_RS01510 327617..328543 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H4I74_RS01515 328540..329817 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
H4I74_RS01520 329814..330581 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
H4I74_RS01525 330583..331296 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
H4I74_RS01530 331460..331681 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H4I74_RS01535 331678..332046 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H4I74_RS01540 332319..333635 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
H4I74_RS01545 333742..334629 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
H4I74_RS01550 334626..335471 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
H4I74_RS01555 335473..336543 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327617..337280 9663


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T173247 WP_002920800.1 NZ_CP061957:331678-332046 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T173247 NZ_CP080121:2508951-2509058 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT173247 WP_002920802.1 NZ_CP061957:331460-331681 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT173247 NZ_CP080121:c2508904-2508838 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References