Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 326700..327286 Replicon chromosome
Accession NZ_CP061952
Organism Klebsiella pneumoniae strain IR5654

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag H4I75_RS01520 Protein ID WP_002920800.1
Coordinates 326918..327286 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag H4I75_RS01515 Protein ID WP_002920802.1
Coordinates 326700..326921 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I75_RS01495 322857..323783 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
H4I75_RS01500 323780..325057 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
H4I75_RS01505 325054..325821 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
H4I75_RS01510 325823..326536 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
H4I75_RS01515 326700..326921 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H4I75_RS01520 326918..327286 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H4I75_RS01525 327559..328875 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
H4I75_RS01530 328982..329869 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
H4I75_RS01535 329866..330711 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
H4I75_RS01540 330713..331783 + 1071 WP_129028782.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 311932..332520 20588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T173226 WP_002920800.1 NZ_CP061952:326918-327286 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T173226 NZ_CP080120:2991717-2991824 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT173226 WP_002920802.1 NZ_CP061952:326700-326921 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT173226 NZ_CP080120:c2991660-2991605 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References