Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2250428..2251078 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag IE970_RS10870 Protein ID WP_014227314.1
Coordinates 2250737..2251078 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag IE970_RS10865 Protein ID WP_014227313.1
Coordinates 2250428..2250727 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS10845 2246313..2247032 - 720 WP_032718745.1 phosphonate utilization transcriptional regulator PhnR -
IE970_RS10850 2247173..2248276 + 1104 WP_048262986.1 2-aminoethylphosphonate--pyruvate transaminase -
IE970_RS10855 2248286..2249095 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
IE970_RS10860 2249128..2250309 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
IE970_RS10865 2250428..2250727 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
IE970_RS10870 2250737..2251078 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IE970_RS10875 2251329..2251781 - 453 WP_032718748.1 DUF1198 domain-containing protein -
IE970_RS10880 2251959..2253350 - 1392 WP_014227315.1 hexose-6-phosphate:phosphate antiporter -
IE970_RS10885 2253496..2254815 - 1320 WP_032751824.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T173177 WP_014227314.1 NZ_CP061930:c2251078-2250737 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T173177 NZ_CP080118:2667335-2667586 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT173177 WP_014227313.1 NZ_CP061930:c2250727-2250428 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT173177 NZ_CP080118:2667583-2667837 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References