Detailed information of TA system TA173171

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66930..67183
• Replicon: plasmid p1
• Accession: NZ_CP061915
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IE857_RS23990
• Protein ID: WP_001312851.1
• Coordinates: 67034..67183 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66930..66988 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS23960	62079..62639	+	561	WP_021569950.1	fertility inhibition protein FinO	-
IE857_RS23965	64055..65008	+	954	Protein_84	YadA-like family protein	-
IE857_RS23970	65010..65471	+	462	WP_021553165.1	thermonuclease family protein	-
IE857_RS23975	65517..65726	+	210	WP_137545870.1	hemolysin expression modulator Hha	-
IE857_RS23980	65764..66450	+	687	WP_097178542.1	DUF2726 domain-containing protein	-
IE857_RS23985	66447..66755	+	309	WP_100033842.1	hypothetical protein	-
-	66930..66988	-	59	NuclAT_1	-	Antitoxin
-	66930..66988	-	59	NuclAT_1	-	Antitoxin
-	66930..66988	-	59	NuclAT_1	-	Antitoxin
-	66930..66988	-	59	NuclAT_1	-	Antitoxin
IE857_RS23990	67034..67183	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IE857_RS23995	67467..67724	+	258	WP_000083826.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68025	68025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T173171 WP_001312851.1 NZ_CP061915:67034-67183 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T173171 NZ_CP080118:1067942-1068469 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT173171 NZ_CP061915:c66988-66930 [Escherichia coli]
AGGAACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin