Detailed information of TA system TA173167

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22310..22736
• Replicon: plasmid p1
• Accession: NZ_CP061915
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IE857_RS23715
• Protein ID: WP_001312861.1
• Coordinates: 22578..22736 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22310..22534 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS23685	18011..18217	+	207	WP_000547971.1	hypothetical protein	-
IE857_RS23690	18243..18764	+	522	WP_191596542.1	single-stranded DNA-binding protein	-
IE857_RS23695	18822..19055	+	234	WP_001478623.1	DUF905 family protein	-
IE857_RS23700	19115..21079	+	1965	WP_191596543.1	ParB/RepB/Spo0J family partition protein	-
IE857_RS23705	21148..21582	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IE857_RS23710	21579..22298	+	720	WP_191596544.1	plasmid SOS inhibition protein A	-
-	22310..22534	+	225	NuclAT_0	-	Antitoxin
-	22310..22534	+	225	NuclAT_0	-	Antitoxin
-	22310..22534	+	225	NuclAT_0	-	Antitoxin
-	22310..22534	+	225	NuclAT_0	-	Antitoxin
IE857_RS23715	22578..22736	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IE857_RS23720	23037..23333	-	297	Protein_35	hypothetical protein	-
IE857_RS23725	23403..23606	+	204	Protein_36	single-stranded DNA-binding protein	-
IE857_RS23730	23629..23952	+	324	WP_000533253.1	hypothetical protein	-
IE857_RS23735	24011..24253	+	243	WP_137545882.1	hypothetical protein	-
IE857_RS23740	24946..25152	+	207	WP_000547974.1	hypothetical protein	-
IE857_RS23745	25176..25472	+	297	WP_001272251.1	hypothetical protein	-
IE857_RS23750	25583..26404	+	822	WP_021553189.1	DUF945 domain-containing protein	-
IE857_RS23755	26700..27209	-	510	WP_021553190.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68025	68025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T173167 WP_001312861.1 NZ_CP061915:22578-22736 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T173167 NZ_CP080118:c504415-504104 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT173167 NZ_CP061915:22310-22534 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGACTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCATGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin