Detailed information of TA system TA173165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4751420..4752022
• Replicon: chromosome
• Accession: NZ_CP061914
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IE857_RS22595
• Protein ID: WP_000897305.1
• Coordinates: 4751711..4752022 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IE857_RS22590
• Protein ID: WP_000356397.1
• Coordinates: 4751420..4751710 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS22565	4747346..4748248	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IE857_RS22570	4748245..4748880	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IE857_RS22575	4748877..4749806	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
IE857_RS22580	4750136..4750378	-	243	WP_001087409.1	hypothetical protein	-
IE857_RS22585	4750597..4750815	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IE857_RS22590	4751420..4751710	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IE857_RS22595	4751711..4752022	-	312	WP_000897305.1	hypothetical protein	Toxin
IE857_RS22600	4752251..4753159	+	909	WP_001162704.1	alpha/beta hydrolase	-
IE857_RS22605	4753223..4754164	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IE857_RS22610	4754209..4754646	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IE857_RS22615	4754643..4755515	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IE857_RS22620	4755509..4756108	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T173165 WP_000897305.1 NZ_CP061914:c4752022-4751711 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T173165 NZ_CP080118:c84093-83743 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT173165 WP_000356397.1 NZ_CP061914:c4751710-4751420 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT173165 NZ_CP080118:c84338-84087 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7