Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3917351..3917892 Replicon chromosome
Accession NZ_CP061914
Organism Escherichia coli strain SMBL

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IE857_RS18840 Protein ID WP_000615976.1
Coordinates 3917614..3917892 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IE857_RS18835 Protein ID WP_000729704.1
Coordinates 3917351..3917611 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE857_RS18810 3912387..3913526 + 1140 WP_032216948.1 flagellar type III secretion system protein FlhB -
IE857_RS18815 3913510..3915603 + 2094 WP_047658924.1 flagellar biosynthesis protein FlhA -
IE857_RS18820 3915708..3915986 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IE857_RS18825 3915979..3916335 + 357 WP_191596361.1 type II toxin-antitoxin system HicB family antitoxin -
IE857_RS18830 3916392..3917165 - 774 WP_000543899.1 C40 family peptidase -
IE857_RS18835 3917351..3917611 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IE857_RS18840 3917614..3917892 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IE857_RS18845 3918048..3918788 + 741 WP_001225679.1 murein L,D-transpeptidase -
IE857_RS18850 3918759..3919526 - 768 WP_000333380.1 class II glutamine amidotransferase -
IE857_RS18855 3919732..3920310 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T173163 WP_000615976.1 NZ_CP061914:3917614-3917892 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T173163 NZ_CP080117:c4499417-4499244 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT173163 WP_000729704.1 NZ_CP061914:3917351-3917611 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT173163 NZ_CP080117:c4499741-4499445 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References