Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1946194..1946696 Replicon chromosome
Accession NZ_CP061914
Organism Escherichia coli strain SMBL

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IE857_RS09245 Protein ID WP_000767829.1
Coordinates 1946442..1946696 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IE857_RS09240 Protein ID WP_001259255.1
Coordinates 1946194..1946445 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE857_RS09215 1941372..1942439 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IE857_RS09220 1942439..1943509 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
IE857_RS09225 1943506..1944810 - 1305 WP_191596480.1 histidinol dehydrogenase -
IE857_RS09230 1944816..1945715 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IE857_RS09235 1945861..1945911 - 51 WP_001364200.1 his operon leader peptide -
IE857_RS09240 1946194..1946445 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IE857_RS09245 1946442..1946696 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IE857_RS09250 1946779..1947603 + 825 WP_000754752.1 SDR family oxidoreductase -
IE857_RS09255 1947649..1948578 + 930 WP_000803351.1 LysR family transcriptional regulator -
IE857_RS09260 1948793..1948855 + 63 WP_010723108.1 membrane protein YoeI -
IE857_RS09265 1948845..1950203 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IE857_RS09270 1950382..1951440 + 1059 WP_023568741.1 transport protein YeeE -
IE857_RS09275 1951454..1951681 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T173150 WP_000767829.1 NZ_CP061914:1946442-1946696 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T173150 NZ_CP080117:1911185-1911436 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT173150 WP_001259255.1 NZ_CP061914:1946194-1946445 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT173150 NZ_CP080117:1911433-1911687 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References