Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611395..612194 Replicon chromosome
Accession NZ_CP061914
Organism Escherichia coli strain SMBL

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag IE857_RS02975 Protein ID WP_000347267.1
Coordinates 611395..611859 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IE857_RS02980 Protein ID WP_001307405.1
Coordinates 611859..612194 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE857_RS02945 606396..606830 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IE857_RS02950 606848..607726 - 879 WP_001380079.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IE857_RS02955 607716..608495 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IE857_RS02960 608506..608979 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IE857_RS02965 609002..610282 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IE857_RS02970 610531..611340 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IE857_RS02975 611395..611859 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IE857_RS02980 611859..612194 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IE857_RS02985 612343..613914 - 1572 WP_001273741.1 galactarate dehydratase -
IE857_RS02990 614289..615623 + 1335 WP_191596403.1 galactarate/glucarate/glycerate transporter GarP -
IE857_RS02995 615639..616409 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T173145 WP_000347267.1 NZ_CP061914:c611859-611395 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T173145 NZ_CP080117:295571-295678 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT173145 WP_001307405.1 NZ_CP061914:c612194-611859 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT173145 NZ_CP080117:c295514-295457 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References