Detailed information of TA system TA173105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 470143..470730
• Replicon: chromosome
• Accession: NZ_CP061847
• Organism: Lysobacter sp. CW239

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0A0ES02
• Locus tag: H2514_RS01965
• Protein ID: WP_036192000.1
• Coordinates: 470143..470424 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2514_RS01970
• Protein ID: WP_036192002.1
• Coordinates: 470434..470730 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2514_RS01950	465635..466498	-	864	WP_036191991.1	A24 family peptidase	-
H2514_RS01955	466558..467820	-	1263	WP_036191993.1	type II secretion system F family protein	-
H2514_RS01960	468137..469864	-	1728	WP_036191997.1	type IV-A pilus assembly ATPase PilB	-
H2514_RS01965	470143..470424	+	282	WP_036192000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2514_RS01970	470434..470730	+	297	WP_036192002.1	HigA family addiction module antidote protein	Antitoxin
H2514_RS01975	470776..471236	-	461	Protein_389	DUF1643 domain-containing protein	-
H2514_RS01980	471556..472014	-	459	WP_036192005.1	pilin	-
H2514_RS01985	472450..472728	+	279	WP_036192015.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H2514_RS01990	472747..473028	+	282	WP_036192407.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (11-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10633.18 Da
• Isoelectric Point: 8.2868

>T173105 WP_036192000.1 NZ_CP061847:470143-470424 [Lysobacter sp. CW239]
MSIQSFQCAETESFFNGRTVKKFEGFKKVAMRKLAMLDAAKALNDLRVPPANRLEPLRGDREGQHSIRVNDQFRVCFIWA
DAGPTNVEICDYH

Nucleotide

• Length: 282 bp

>T173105 NZ_CP080100:c199925-199443 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10917.52 Da
• Isoelectric Point: 6.0641

>AT173105 WP_036192002.1 NZ_CP061847:470434-470730 [Lysobacter sp. CW239]
MRSVPYPHPGEILLEEFLVPLGISQYKLAKAIDVPQTRISAIIAGRRSITADTALRLARFFSMSDSFWMNLQVGHDAAQA
RDELADVLAHIEPYEHAA

Nucleotide

• Length: 297 bp

>AT173105 NZ_CP080100:c200179-199913 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0A0ES02