Detailed information of TA system TA173058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 95332..95983
• Replicon: plasmid pAS42-2
• Accession: NZ_CP061830
• Organism: Acinetobacter seifertii strain AS42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC779_RS21040
• Protein ID: WP_006581604.1
• Coordinates: 95332..95688 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC779_RS21045
• Protein ID: WP_001140619.1
• Coordinates: 95681..95983 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC779_RS21020	90789..91997	-	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-
IC779_RS21025	92282..92458	+	177	WP_006581600.1	hypothetical protein	-
IC779_RS21030	92492..92854	+	363	Protein_96	DNA-binding protein	-
IC779_RS21035	93068..94522	-	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC779_RS21040	95332..95688	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC779_RS21045	95681..95983	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC779_RS21050	95976..96185	+	210	WP_000069471.1	hypothetical protein	-
IC779_RS21055	96295..96513	-	219	WP_029747731.1	hypothetical protein	-
IC779_RS21065	98423..98736	-	314	Protein_103	AAA family ATPase	-
IC779_RS21070	98922..99242	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC779_RS21075	99519..99707	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC779_RS21080	99694..99983	+	290	Protein_106	helix-turn-helix domain-containing protein	-
IC779_RS21085	100062..100316	-	255	WP_000236586.1	hypothetical protein	-
IC779_RS21090	100267..100564	-	298	Protein_108	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105816	105816
	-	flank	IS/Tn	-	-	90789..91997	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T173058 WP_006581604.1 NZ_CP061830:95332-95688 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T173058 NZ_CP080085:c4190736-4190518 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT173058 WP_001140619.1 NZ_CP061830:95681-95983 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT173058 NZ_CP080085:c4191136-4190762 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0