Detailed information of TA system TA173018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 144773..145376
• Replicon: chromosome
• Accession: NZ_CP061800
• Organism: Desulfonema magnum strain 4be13

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: dnm_RS00620
• Protein ID: WP_207683554.1
• Coordinates: 145095..145376 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: dnm_RS00615
• Protein ID: WP_207680789.1
• Coordinates: 144773..145081 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
dnm_RS00595 (dnm_001740)	140247..142214	-	1968	WP_207680785.1	hypothetical protein	-
dnm_RS00600 (dnm_001770)	142576..143133	-	558	WP_207680786.1	hypothetical protein	-
dnm_RS00605 (dnm_001780)	143145..144272	-	1128	WP_207680787.1	AAA family ATPase	-
dnm_RS00610 (dnm_001790)	144399..144710	-	312	WP_207680788.1	HigA family addiction module antitoxin	-
dnm_RS00615 (dnm_001800)	144773..145081	-	309	WP_207680789.1	HigA family addiction module antitoxin	Antitoxin
dnm_RS00620 (dnm_001810)	145095..145376	-	282	WP_207683554.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
dnm_RS00625	145540..146645	+	1106	WP_207680790.1	IS630 family transposase	-
dnm_RS00630 (dnm_001850)	146899..147291	-	393	WP_207680791.1	hypothetical protein	-
dnm_RS00635 (dnm_001860)	147454..148914	-	1461	WP_207680792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-82)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11224.87 Da
• Isoelectric Point: 10.3115

>T173018 WP_207683554.1 NZ_CP061800:c145376-145095 [Desulfonema magnum]
MIKSFKDKETEKIFCLRRSRRLPPEIQQTALRKLRMLNNAQNLQDLRVPPANRLEKLTKDRAGQHSIRINDQWRICFEWR
NGDAYEAEITDYH

Nucleotide

• Length: 282 bp

>T173018 NZ_CP080077:12488-12769 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.88 Da
• Isoelectric Point: 4.3766

>AT173018 WP_207680789.1 NZ_CP061800:c145081-144773 [Desulfonema magnum]
MTQDKLNPVHPGEILAEEFLKPMGLTPEQLASDIGVNITQLDKIIAEKEGMNADMALRLARYFGTSPRFWTGLQADYDLD
VTSEYLGNRLEQEVKPRAVAAG

Nucleotide

• Length: 309 bp

>AT173018 NZ_CP080077:12247-12498 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA