Detailed information of TA system TA173015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5102157..5102780
• Replicon: chromosome
• Accession: NZ_CP061799
• Organism: Desulfonema limicola strain 5ac10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: dnl_RS21735
• Protein ID: WP_207688319.1
• Coordinates: 5102502..5102780 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: dnl_RS21730
• Protein ID: WP_207688318.1
• Coordinates: 5102157..5102492 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
dnl_RS21715 (dnl_47500)	5098044..5099633	+	1590	WP_207687548.1	hypothetical protein	-
dnl_RS21720 (dnl_47510)	5099885..5101387	+	1503	WP_207687720.1	IS1380 family transposase	-
dnl_RS21725 (dnl_47520)	5101601..5102116	-	516	WP_207688317.1	DUF1156 domain-containing protein	-
dnl_RS21730 (dnl_47530)	5102157..5102492	-	336	WP_207688318.1	HigA family addiction module antitoxin	Antitoxin
dnl_RS21735 (dnl_47540)	5102502..5102780	-	279	WP_207688319.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
dnl_RS21740 (dnl_47550)	5102808..5106014	-	3207	WP_207688320.1	helicase-related protein	-
dnl_RS21745 (dnl_47560)	5105948..5106301	-	354	WP_207688321.1	hypothetical protein	-
dnl_RS21750 (dnl_47570)	5106497..5106697	-	201	WP_207688322.1	PDDEXK nuclease domain-containing protein	-
dnl_RS21755 (dnl_47575)	5106708..5107115	-	408	WP_207688323.1	DUF1016 N-terminal domain-containing protein	-
dnl_RS21760 (dnl_47580)	5107223..5107642	-	420	WP_207688324.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10784.23 Da
• Isoelectric Point: 9.5920

>T173015 WP_207688319.1 NZ_CP061799:c5102780-5102502 [Desulfonema limicola]
MIKSFKHKGLEDFFYTGSKRGIKPEHANKLERILDRLNAASDIKDMNYPGSFLHQLTGDKKGFYSVRVSGNWRIFFKFID
GDAYIVNYDDYH

Nucleotide

• Length: 279 bp

>T173015 NZ_CP080075:c4245128-4244955 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12750.83 Da
• Isoelectric Point: 9.9638

>AT173015 WP_207688318.1 NZ_CP061799:c5102492-5102157 [Desulfonema limicola]
MRKMIRHPIHPGNILKQDYLLPLSLTIKDMAANLGVSTKTLSRIINEKGSVTPDMALRLSRAFDTTPDLWLNLQKNYDLW
QAETISQEWKKVKPFPQHILDSPSLNSEQIV

Nucleotide

• Length: 336 bp

>AT173015 NZ_CP080075:c4245452-4245156 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG