Detailed information of TA system TA173014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4238533..4239848
• Replicon: chromosome
• Accession: NZ_CP061799
• Organism: Desulfonema limicola strain 5ac10

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: dnl_RS18145
• Protein ID: WP_207687648.1
• Coordinates: 4238533..4239540 (-)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: dnl_RS18150
• Protein ID: WP_207687649.1
• Coordinates: 4239537..4239848 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
dnl_RS18145 (dnl_39790)	4238533..4239540	-	1008	WP_207687648.1	HipA domain-containing protein	Toxin
dnl_RS18150 (dnl_39800)	4239537..4239848	-	312	WP_207687649.1	HipA N-terminal domain-containing protein	Antitoxin
dnl_RS18155 (dnl_39810)	4239835..4240095	-	261	WP_207687650.1	helix-turn-helix domain-containing protein	-
dnl_RS18160 (dnl_39820)	4240187..4241974	-	1788	WP_207687651.1	ATP-binding protein	-
dnl_RS18165 (dnl_39830)	4242166..4242807	+	642	WP_207687652.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (51-281)

Antitoxin

• HipA, N-terminal subdomain 1 (4-99)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 37875.20 Da
• Isoelectric Point: 9.9713

>T173014 WP_207687648.1 NZ_CP061799:c4239540-4238533 [Desulfonema limicola]
MNICPITYSACDGRYSKAGLKLFSRNLSELNDLPFTAEEQIREAAARASKMSIQGVQPKLSAVLNTRKQVFELVDINGQF
ILKPQNPMYPELPENEDLTMRLAKLSGIEVPLHSLLYSKDGSLTYCIKRFDRLPGGKKAAVEDFSQLMGLTRETKYNTSM
EKVAAVTDRFCTFPAVEKVKLFRLTLFNFLTGNEDMHVKNFSLLTKPGRSKPDKIELSPAYDLLNTSIAIGKPAEETALP
IKAKKNKITREILADYFAGERLGLNDKVISSVIENIKSVLPAWNELINISFLSNKMKDKYKLLVNKRIDRIFGIGYHLAQ
LKVPEVRKSLQKYFI

Nucleotide

• Length: 1008 bp

>T173014 NZ_CP080075:c4142231-4141881 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11657.55 Da
• Isoelectric Point: 7.0117

>AT173014 WP_207687649.1 NZ_CP061799:c4239848-4239537 [Desulfonema limicola]
MRKAEVFFHNISAGILEETEPGKAYRFIYKTDYSGPPVSLTMPVKNSIYEFNRFPSFFEGLLPEGLQLESLLRIKKIDRN
DLFSQLMAVGADVIGAAVIKEIK

Nucleotide

• Length: 312 bp

>AT173014 NZ_CP080075:c4142476-4142225 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA