Detailed information of TA system TA173006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/VapI(antitoxin)
• Location: 160101..161530
• Replicon: chromosome
• Accession: NZ_CP061799
• Organism: Desulfonema limicola strain 5ac10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: dnl_RS00590
• Protein ID: WP_207689846.1
• Coordinates: 161195..161530 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: dnl_RS00585
• Protein ID: WP_207689845.1
• Coordinates: 160101..161195 (-)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
dnl_RS00575 (dnl_01280)	155913..158930	-	3018	WP_207689843.1	ATP-binding protein	-
dnl_RS00580 (dnl_01290)	158927..159850	-	924	WP_207689844.1	hypothetical protein	-
dnl_RS00585 (dnl_01300)	160101..161195	-	1095	WP_207689845.1	HigA family addiction module antitoxin	Antitoxin
dnl_RS00590 (dnl_01310)	161195..161530	-	336	WP_207689846.1	killer suppression protein	Toxin
dnl_RS00595 (dnl_01320)	161773..162000	-	228	WP_207689847.1	M48 family metallopeptidase	-
dnl_RS00600 (dnl_01330)	161961..162386	-	426	WP_207689848.1	DUF45 domain-containing protein	-
dnl_RS00610	162760..162981	-	222	WP_207689849.1	hypothetical protein	-
dnl_RS00615 (dnl_01350)	163011..163640	-	630	WP_207689850.1	DUF2284 domain-containing protein	-
dnl_RS00620 (dnl_01360)	163855..164412	+	558	WP_207689851.1	response regulator	-
dnl_RS00625 (dnl_01370)	164432..165190	+	759	WP_207689852.1	ParA family protein	-
dnl_RS00630 (dnl_01380)	165193..165612	+	420	WP_207689853.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-73)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12918.16 Da
• Isoelectric Point: 9.8032

>T173006 WP_207689846.1 NZ_CP061799:c161530-161195 [Desulfonema limicola]
MDITFKTKKLKKIFNNSKLLIKEYGEQTADKIQIRMIVLRSANNLDLVPKTPPDRCHQLTNNRKETFAVDLKHPFRLIFK
PDMEEIPKLSDGGIDLKEINVIKILGVEDYH

Nucleotide

• Length: 336 bp

>T173006 NZ_CP080075:c2388690-2388514 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 42444.27 Da
• Isoelectric Point: 9.8987

>AT173006 WP_207689845.1 NZ_CP061799:c161195-160101 [Desulfonema limicola]
MNYLNQFNPDYAIHPGVYIEEILEARKMKKSDFAERCGISSKTISQIINKKVLFSADAAVRFEKVLGVNADLLMNMSTSY
QMFEIREGKKNELKKKAEWLKKFPYKELIKFNIIQSSNDISDLANSLLDFFNVSSPSIWDEYYSNKAISFRKSKNYKSSL
HSTMTWLRIGEKLSEEIECKNFNIGVFKENLKEIKKLTKLSLNKFEPEIKKLCNDSGVALVLIPEFKGMRISGITEWLTP
KKALILMSLRYKTNDHFWFTFFHEAGHIILHNKKDIFIDGIDNKDISDIEKEADVFARKTLINQANYDKFVESKKFYKNN
IVKFANQNNIAPGIIVGMLQHDKLINYSWHNNLKEKFPLQLKLK

Nucleotide

• Length: 1095 bp

>AT173006 NZ_CP080075:c2388468-2388052 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA