Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1538378..1538973 Replicon chromosome
Accession NZ_CP061780
Organism Pseudomonas aeruginosa strain ZBX-P11

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag IFJ77_RS07310 Protein ID WP_003113526.1
Coordinates 1538695..1538973 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag IFJ77_RS07305 Protein ID WP_003111575.1
Coordinates 1538378..1538683 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFJ77_RS07270 (IFJ77_07270) 1533530..1534378 + 849 WP_009878189.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
IFJ77_RS07280 (IFJ77_07280) 1534545..1535486 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
IFJ77_RS07285 (IFJ77_07285) 1535603..1536217 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
IFJ77_RS07290 (IFJ77_07290) 1536258..1536842 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
IFJ77_RS07295 (IFJ77_07295) 1536883..1537983 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
IFJ77_RS07305 (IFJ77_07305) 1538378..1538683 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
IFJ77_RS07310 (IFJ77_07310) 1538695..1538973 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IFJ77_RS31290 1539026..1539154 - 129 Protein_1445 integrase -
IFJ77_RS07315 (IFJ77_07315) 1539302..1541530 + 2229 WP_021264080.1 TonB-dependent receptor -
IFJ77_RS07320 (IFJ77_07320) 1541600..1542247 - 648 WP_003095021.1 carbonate dehydratase -
IFJ77_RS07325 (IFJ77_07325) 1542309..1543547 - 1239 WP_003111578.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T173002 WP_003113526.1 NZ_CP061780:c1538973-1538695 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T173002 NZ_CP080075:1780215-1780466 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT173002 WP_003111575.1 NZ_CP061780:c1538683-1538378 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT173002 NZ_CP080075:1780463-1780717 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATATCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References