Detailed information of TA system TA172998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4858307..4858902
• Replicon: chromosome
• Accession: NZ_CP061779
• Organism: Pseudomonas aeruginosa strain ZBX-P12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IFJ78_RS22820
• Protein ID: WP_003113526.1
• Coordinates: 4858624..4858902 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IFJ78_RS22815
• Protein ID: WP_003113527.1
• Coordinates: 4858307..4858612 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ78_RS22780 (IFJ78_22775)	4853458..4854306	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
IFJ78_RS22790 (IFJ78_22785)	4854473..4855414	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
IFJ78_RS22795 (IFJ78_22790)	4855531..4856145	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
IFJ78_RS22800 (IFJ78_22795)	4856187..4856771	+	585	WP_033942324.1	aminoacyl-tRNA hydrolase	-
IFJ78_RS22805 (IFJ78_22800)	4856812..4857912	+	1101	WP_033942321.1	redox-regulated ATPase YchF	-
IFJ78_RS22815 (IFJ78_22810)	4858307..4858612	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IFJ78_RS22820 (IFJ78_22815)	4858624..4858902	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IFJ78_RS33245	4858955..4859083	-	129	Protein_4534	integrase	-
IFJ78_RS22825 (IFJ78_22820)	4859231..4861459	+	2229	WP_033942306.1	TonB-dependent receptor	-
IFJ78_RS22830 (IFJ78_22825)	4861529..4862176	-	648	WP_003095021.1	carbonate dehydratase	-
IFJ78_RS22835 (IFJ78_22830)	4862238..4863476	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T172998 WP_003113526.1 NZ_CP061779:c4858902-4858624 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T172998 NZ_CP080075:c615636-615172 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT172998 WP_003113527.1 NZ_CP061779:c4858612-4858307 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT172998 NZ_CP080075:c615971-615636 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V6ALY3