Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 448969..449564 Replicon chromosome
Accession NZ_CP061778
Organism Pseudomonas aeruginosa strain ZBX-P13

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag IFJ79_RS02200 Protein ID WP_003113526.1
Coordinates 449286..449564 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag IFJ79_RS02195 Protein ID WP_003111575.1
Coordinates 448969..449274 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFJ79_RS02170 (IFJ79_02165) 444766..445557 + 792 WP_042855636.1 hypothetical protein -
IFJ79_RS02175 (IFJ79_02170) 445504..445809 - 306 WP_071567699.1 GIY-YIG nuclease family protein -
IFJ79_RS02180 (IFJ79_02175) 445802..447136 - 1335 WP_161397126.1 hypothetical protein -
IFJ79_RS02185 (IFJ79_02180) 447111..447584 - 474 WP_003148665.1 helix-turn-helix transcriptional regulator -
IFJ79_RS02190 (IFJ79_02185) 447591..448781 - 1191 WP_023442562.1 tyrosine-type recombinase/integrase -
IFJ79_RS02195 (IFJ79_02190) 448969..449274 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
IFJ79_RS02200 (IFJ79_02195) 449286..449564 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IFJ79_RS31795 449617..449745 - 129 Protein_433 integrase -
IFJ79_RS02205 (IFJ79_02200) 449893..452121 + 2229 WP_033991161.1 TonB-dependent receptor -
IFJ79_RS02210 (IFJ79_02205) 452192..452839 - 648 WP_003095021.1 carbonate dehydratase -
IFJ79_RS02215 (IFJ79_02210) 452901..454139 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T172985 WP_003113526.1 NZ_CP061778:c449564-449286 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T172985 NZ_CP080070:1025761-1026096 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT172985 WP_003111575.1 NZ_CP061778:c449274-448969 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT172985 NZ_CP080070:1025513-1025761 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References