Detailed information of TA system TA172982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5699656..5700251
• Replicon: chromosome
• Accession: NZ_CP061777
• Organism: Pseudomonas aeruginosa strain ZBX-P23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IFJ80_RS26940
• Protein ID: WP_003113526.1
• Coordinates: 5699973..5700251 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IFJ80_RS26935
• Protein ID: WP_003113527.1
• Coordinates: 5699656..5699961 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ80_RS26900 (IFJ80_26895)	5694807..5695655	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
IFJ80_RS26910 (IFJ80_26905)	5695822..5696763	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
IFJ80_RS26915 (IFJ80_26910)	5696880..5697494	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
IFJ80_RS26920 (IFJ80_26915)	5697536..5698120	+	585	WP_033942324.1	aminoacyl-tRNA hydrolase	-
IFJ80_RS26925 (IFJ80_26920)	5698161..5699261	+	1101	WP_033942321.1	redox-regulated ATPase YchF	-
IFJ80_RS26935 (IFJ80_26930)	5699656..5699961	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
IFJ80_RS26940 (IFJ80_26935)	5699973..5700251	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IFJ80_RS33705	5700304..5700432	-	129	Protein_5348	integrase	-
IFJ80_RS26945 (IFJ80_26940)	5700580..5702808	+	2229	WP_033942306.1	TonB-dependent receptor	-
IFJ80_RS26950 (IFJ80_26945)	5702878..5703525	-	648	WP_003095021.1	carbonate dehydratase	-
IFJ80_RS26955 (IFJ80_26950)	5703587..5704825	-	1239	WP_033942304.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T172982 WP_003113526.1 NZ_CP061777:c5700251-5699973 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T172982 NZ_CP080070:701117-701431 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT172982 WP_003113527.1 NZ_CP061777:c5699961-5699656 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT172982 NZ_CP080070:701428-701844 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACACTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: V6ALY3