Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1932037..1932539 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ID627_RS09290 Protein ID WP_000767829.1
Coordinates 1932285..1932539 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ID627_RS09285 Protein ID WP_001259255.1
Coordinates 1932037..1932288 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS09260 1927215..1928282 - 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
ID627_RS09265 1928282..1929352 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
ID627_RS09270 1929349..1930653 - 1305 WP_001559366.1 histidinol dehydrogenase -
ID627_RS09275 1930659..1931558 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
ID627_RS09280 1931704..1931754 - 51 WP_001364200.1 his operon leader peptide -
ID627_RS09285 1932037..1932288 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ID627_RS09290 1932285..1932539 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ID627_RS09295 1932622..1933446 + 825 WP_000754758.1 SDR family oxidoreductase -
ID627_RS09300 1933492..1934421 + 930 WP_000803360.1 LysR family transcriptional regulator -
ID627_RS09305 1934636..1934698 + 63 WP_010723108.1 membrane protein YoeI -
ID627_RS09310 1934688..1936046 + 1359 WP_053274921.1 putrescine/proton symporter PlaP -
ID627_RS09315 1936225..1937283 + 1059 WP_000492305.1 transport protein YeeE -
ID627_RS09320 1937297..1937524 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T172958 WP_000767829.1 NZ_CP061764:1932285-1932539 [Escherichia coli O19:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T172958 NZ_CP080066:209186-209293 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT172958 WP_001259255.1 NZ_CP061764:1932037-1932288 [Escherichia coli O19:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT172958 NZ_CP080066:c209129-209074 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References