Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1180303..1181030 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag ID627_RS05775 Protein ID WP_000547564.1
Coordinates 1180303..1180614 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ID627_RS05780 Protein ID WP_000126294.1
Coordinates 1180611..1181030 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS05745 1175445..1177154 + 1710 WP_191113493.1 formate hydrogenlyase subunit HycE -
ID627_RS05750 1177164..1177706 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
ID627_RS05755 1177706..1178473 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
ID627_RS05760 1178470..1178880 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
ID627_RS05765 1178873..1179343 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
ID627_RS05770 1179368..1180129 + 762 WP_001026446.1 hypothetical protein -
ID627_RS05775 1180303..1180614 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
ID627_RS05780 1180611..1181030 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
ID627_RS05785 1181144..1182568 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
ID627_RS05790 1182577..1184034 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
ID627_RS05795 1184294..1185304 + 1011 WP_032148643.1 DNA-binding transcriptional regulator AscG -
ID627_RS05800 1185453..1185980 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T172957 WP_000547564.1 NZ_CP061764:1180303-1180614 [Escherichia coli O19:H7]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T172957 NZ_CP080066:208703-208810 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT172957 WP_000126294.1 NZ_CP061764:1180611-1181030 [Escherichia coli O19:H7]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT172957 NZ_CP080066:c208645-208591 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References